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The ability to add the CellEvent Caspase-3/7 Green Detection Reagent directly to the live cells in the medium without the need to wash, fix, or permeabilize the cells is ideal for this endpoint assay experiment. Cell toxicity was measured using the fluorogenic substrate Invitrogen CellEvent Caspase-3/7 Green Detection Reagent, which reacts with the enzymes caspase-3 and caspase-7, which are activated during apoptosis. These cells were evaluated for toxicity using an apoptosis reporter assay and analyzed on the Attune NxT Flow Cytometer. Jurkat cells, comprising human T lymphocyte cells used to study acute T cell leukemia, were grown and treated in 96-well plates with various concentrations of different cancer drugs as described below. This application note discusses the use of the Invitrogen Attune NxT Flow Cytometer with Autosampler to evaluate the effectiveness of several cancer drugs in inducing apoptosis in cancer cells, with dose response curves. The use of flow cytometry enables accurate simultaneous evaluation of multiple analytes, in a statistically relevant sample size in a short time. A Guide to Analyze Attune NxT FCS Data Files in FCS Express 6 SoftwareÄose response curves are a standard research technique used to evaluate therapeutic efficacy of cancer drugs.High-Throughput Automation With the Attune NxT Autosampler: Consistent Results Across All Wells and Across Plates.Validated 10-Color T Cell Panel Using the Attune NxT Flow Cytometer.Recommendations for Accurate Concentration Measurements on the Attune NxT Flow Cytometer.Flow Cytometry Analysis of Transcription Factor Expression During Differentiation of hPSC-Derived Cardiomyocytes.Detection of Platelets in Whole Blood Using the Attune NxT Flow Cytometer.Flow Cytometry Analysis of Dose Response for Apoptosis Induction.Detection of Fluorescent Proteins Using the Attune NxT Flow Cytometer.Recommendations for Accurate Carryover Measurements on the Attune NxT Flow Cytometer.Live/Dead Algal Analysis With the Attune NxT Flow Cytometer.No-Wash, No-Lyse Detection of Phagocytic Cells Via a pHrodo BioParticles Functional Assay in Human Whole Blood on the Attune NxT Flow Cytometer.No-Wash, No-Lyse Detection of Leukocytes In Human Whole Blood on the Attune NxT Flow Cytometer.13-Parameter Immunophenotyping of Human Lysed Whole Blood With the Attune NxT Flow Cytometer Allows for the Identification of B cells, NK cells, Multiple T Cell Subsets and Myeloid Cells.
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Copper-Safe Click-iT Plus EdU Proliferation Assay: Improved Compatibility with Simultaneous Phycobiliprotein and Fluorescent Protein Detection.Flow Cytometry Multiplexing Used to Analyze Metabolic Activity and Assess Pharmaceutical Compound Toxicity as A High-Throughput Screening Tool.Ten-Parameter Immunophenotyping of Human Lysed Whole Blood With the Attune NxT Flow Cytometer Allows Identification of Multiple T Cell Subsets and Myeloid Cells.Attune NxT Flow Cytometer for 6-Color Immunophenotyping Analysis of Stained Human Whole Blood Using a No-Lyse, No-Wash Protocol, With No Compensation.Multiparameter Analysis of Murine Regulatory T Cells and Dendritic Cells With the Attune NxT Flow Cytometer.Detection of Murine Regulatory T cells on the Attune NxT Flow Cytometer.